Cobalt labelling of single primary auditory neurones: an alternative to HRP.
نویسندگان
چکیده
We have labelled single, primary auditory neurones in three reptile and one bird species. After functional characterization of the neurones, hexamminecobaltic chloride was iontophoretically injected through the recording micropipette. Precipitation of cobalt sulfide followed by silver intensification of the cochlear duct as a whole-mount preparation revealed stained neurones in over 90% of cases. This method has several advantages over labelling with HRP.
منابع مشابه
Membrane properties of histaminergic tuberomammillary neurones of the rat hypothalamus in vitro.
1. Intracellular recordings were obtained from neurones of the tuberomammillary nucleus in an in vitro explant of the rat hypothalamus. 2. Tuberomammillary neurones were spontaneously active (2.1 +/- 0.6 Hz) at the resting potential which was around -50 mV. Action potential amplitude was 75 +/- 8 mV (n = 9); mean mid-amplitude duration was 1.8 +/- 0.4 ms (n = 9). 3. The mean input resistance of...
متن کاملThe infrared trigemino-tectal pathway in the rattlesnake and in the python.
We have studied the infrared trigemino-tectal pathway of the rattlesnake (Crotalus viridis) and the python (P. reticulatus). In the rattlesnake, horseradish perosidase (HRP) injections into the nucleus reticularis caloris (RC) result in retrograde filling of cells in the ipsilateral nucleus of the lateral descending trigeminal tract (LTTD) and in the anterograde labelling of terminal fields in ...
متن کاملDistinctive membrane and discharge properties of rat spinal lamina I projection neurones in vitro.
Most lamina I neurones with a projection to the brainstem express the neurokinin 1 receptor and thus belong to a small subgroup of lamina I neurones that are necessary for the development of hyperalgesia in rat models of persisting pain. These neurones are prone to synaptic plasticity following primary afferent stimulation in the noxious range while other nociceptive lamina I neurones are not. ...
متن کاملThe effects of tectal lesion on the survival of isthmic neurones in Xenopus.
The isthmic nucleus (IN) is a visual relay centre of the frog brain. It receives afferent projection from the optic tectum of the same side and projects bilaterally to both tecta. In young postmetamorphic Xenopus frogs, the survival of neurones in the IN on both sides was studied following the complete removal of the right tectum. In 6- to 8-week-old frogs, the right tectum was surgically remov...
متن کاملAuditory brainstem projections to the ferret superior colliculus: anatomical contribution to the neural coding of sound azimuth.
The mammalian superior colliculus (SC) contains a neural map of auditory space. It is not known whether this topographic representation emerges at the level of the SC or is relayed there from other auditory areas. We have used retrograde labelling techniques in ferrets to examine the sources and pattern of innervation from auditory brainstem nuclei. After multiple injections of wheat germ agglu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Hearing research
دوره 32 2-3 شماره
صفحات -
تاریخ انتشار 1988